Ocular surface reconstruction with tissue-engineered corneal epithelium
Zhang Liying*, Qu Yangluowa, Zou Dulei, Li Juan, Ou Shangkun,Liu Tingting,Dong Nuo,Lin Zhirong,Luo Shunrong,Shang Xumin,Yan Lei,Xie Zhiwen,,Wu Huping, Liu Zuguo, Li Wei
Eye Institute of Xiamen University, Xiamen 361102, China
Objective To investigate the short-term outcome of ocular surface reconstruction with tissue-engineered corneal epithelium in the treatment of total limbal stem cell deficiency (LSCD). Methods In a case series, 6 eyes of 6 patients with total LSCD resulting from severe chemical injury (3 eyes), blast injury (2 eyes), or thermal burn (1 eye) were included in this study. Denuded amniotic membrane was used as the carrier to culture autologous corneal epithelial stem cells to construct tissue-engineered corneal epithelium. After that, tissue-engineered corneal epithelium was transplanted onto the LSCD ocular surface after removal of the corneal pannus. Visual acuity, slit lamp photography, ultrasound biomicroscopy (UBM) and Schirmer I test were performed before surgery and during the follow-up. Results After cell culture for 3 weeks, the autologous corneal epithelial cells proliferated and formed a stratified epithelial cell sheet 15 mm in diameter. All tissue-engineered corneal epithelia were successfully transplanted onto the ocular surface of the LSCD patients. Three months after surgery, there were no epithelial defects in the recipient corneas and the corneal surface remained smooth. Corneal scar tissue was removed and the pannus was significantly reduced. There was an improvement in visual acuity in all patients. Conclusion Transplantation of tissue-engineered corneal epithelia based on the ex-vivo expansion of autologous corneal epithelial stem cells may be an effective treatment to reconstruct the ocular surface of LSCD patients.
张丽颖,瞿杨洛娃,邹笃雷,李娟,欧尚坤,刘婷婷,董诺,林志荣,罗顺荣,商旭敏,闫蕾,谢智文,吴护平,刘祖国,李炜 .组织工程角膜上皮移植重建眼表面的临床研究. 中华眼视光学与视觉科学杂志, 2016, 18(4):209-214. DOI:DOI:10.3760/cma.j.issn.1674-845X.2016.04.004.
Zhang Liying*, Qu Yangluowa, Zou Dulei, Li Juan, Ou Shangkun,Liu Tingting,Dong Nuo,Lin Zhirong,Luo Shunrong,Shang Xumin,Yan Lei,Xie Zhiwen,,Wu Huping, Liu Zuguo, Li Wei.Ocular surface reconstruction with tissue-engineered corneal epithelium. Chinese Journal of Optometry Ophthalmology and Visual science, 2016, 18(4):209-214. DOI:DOI:10.3760/cma.j.issn.1674-845X.2016.04.004.
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