Objective To study the impact on human visual function after viewing 2D/3D television; to explore the corresponding relationship between visual fatigue and human wavefront aberrations. Methods Thirty healthy adults were recruited for this research study. They watched the same film on 2D and 3D television but they wore two types of polarized 3D glasses for the 3D task-type A and type B (the optical index of type A was better than type B). Wavefront aberrations of the subjects was recorded before and after the visual task, included the modulation transfer function (MTF), root mean square (RMS) of higher order aberrations amplitude, etc. A single factor analysis of variance was used to compare values between the groups. Results The MTF variation was maximized when watching 3D television with type B glasses (3D-B), so the MTF tended to diminish after watching the film. The differences between 2D, 3D-A and 3D-B condition were significant (P<0.05). In the three test conditions, there were significant differences in RMS3 and RMS4 comparisons between 3D-A and 3D-B condition (F=0.293, 0.044, P<0.05). Vertical coma variations under the 2D/3D conditions were negative, and there were significant differences between 2D, 3D-A and 3D-B condition (F=0.025, P<0.05). Conclusion The stimulation of 2D/3D film can lead to changes in visual quality, which improves the index change in wavefront aberrations. This can be used in the future for evaluating visual comfort when viewing 3D television.