Objective To investigate the effect of circadian rhythm on the visual electrophysiology of the SD rat. Methods In this experimental study, 24 SD rats were divided into 4 groups randomly, 6 rats (6 eyes) in each group. Visual electrophysiology of the 4 groups was recorded at different time periods respetively with different stimulation parameters: 8:00-12:00 (morning), 14:00-18:00 (afternoon), 20:00-24:00 (midnight), and 2:00-6:00 (before dawn). ANOVA was used to analyze the differences among the groups and post hoc analysis was used to compare the difference between any two groups. Results The a-wave and b-wave amplitudes of dark-adapted electroretinogram (ERG) was higher in the afternoon and midnight than in the morning. The O2 amplitude of the oscillatory potentials was highest at midnight. The latency of the P1 wave of the 20 Hz flicker ERG reaction and the N1 wave of the visual evoked potential during the before draw was significantly shorter than that in the morning. Conclusion The cone and rod functions in SD rats are related to circadian rhythm, and it is more active at night than during the day. It is better to schedule visual electrophysiological recordings in SD rats during the same period of the day.
顾永昊,张磊,安晶,张作明. 似昼夜节律对正常SD大鼠视觉电生理的影响[J]. 中华眼视光学与视觉科学杂志, 2013, 15(6): 327-331.
GU Yong-hao,ZHANG Lei,AN Jing,ZHANG Zuo-ming. Effects of circadian rhythm on the visual electrophysiology of the rats. Chinese Journal of Optometry Ophthalmology and Visual Science, 2013, 15(6): 327-331. DOI: 10.3760/cma.j.issn.1674-845X.2013.06.003
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