Chinese Journal of Optometry Ophthalmology and Visual science

中华眼视光学与视觉科学杂志

Online Review

Email Alert

RSS

Instructions for Authors

Quick Search

Advanced Search

Chinese Journal of Optometry Ophthalmology and Visual science

	2020 Vol.22 Issue.4 Published 2020-04-25

	Orignal Article Case Report Editorial Original Articles Review Lecture

Editorial

	241	2019-nCoV and Eye, What We Know and What We Should Do
		Xiangtian Zhou, Jia Qu
		A high contagious virus—— novel coronavirus (2019-nCoV) is proved to be the pathogen causing a global outbreak of coronavirus disease 2019, which is threatening the global public health. This article preliminarily reviews several different aspects, including the characteristics of 2019-nCoV, the anatomical connection between ocular surface and respiratory tract, previous work on the link between respiratory virus and ocular complication, and the data of angiotension-converting enzyme 2 receptor expression and molecular detection of SARS virus in tear. Based on these reviews, intervention measures are advised for ophthalmic practitioner, whilst suggestions are indicated for further clinical and basic investigations in the future.
		2020 Vol. 22 (4): 241-246 [Abstract] ( 311 ) [HTML 1KB] [ PDF 1140KB] ( 2834 )

Original Articles

	247	The Practice and Effectiveness of 2019-nCoV RNA Detection for Prevention and Control Procedures in Ophthalmic Hospitals
		Meiqin Zheng, Youpei Wang, Yunfeng Gu, Liping Mao, Wencan Wu, Wei Chen, Xinping Yu, Dingxing Ye, Yuanbo Liang, Jia Qu
		Objective: To explore a practical system for precision prevention and control against coronavirus disease 2019 (COVID-19) for eye hospitals by emphasizing systematic and standardized virus RNA detection procedures. Methods: In this case series study, standardized virus RNA detection along with a routine blood test (BRT), C-reactive protein (CRP) and serum amyloid A (SAA) were included in preoperative screening test items for ocular surgical patients and to provide virus RNA detection for returning employees between 24 February 2020 and 2 March 2020. In addition, a questionnaire was designed to investigate the acceptability of nasopharyngeal swab sampling and the influence of virus RNA detection results on the mental status of the examinee. Results: Ninety-nine examination results showed a certain percentage of abnormal test results, among which 13 cases showed an increase in SAA, 12 cases showed a decrease in lymphocyte count (LYM) while 5 cases showed an increase, 1 case showed a decrease in white blood count (WBC) while 11 cases showed an increase, and 2 cases showed an increase in CRP. All cases tested negative for novel coronavirus (2019-nCoV) RNA detection, including examined patients and 33 employees of the Eye Hospital. 11.7% of respondents reported a low acceptance score for nasopharyngeal swab sampling. The proportion of the examinees with higher mental stress scores before and after the virus RNA detection test was 46.7% and 6.7%, respectively. Conclusions: The detection procedures can offer an objective basis for preoperative screening and differential diagnosis, while largely eliminating the possibility of transmission via asymptomatic infected individuals. On the other hand, the negative result of virus RNA detection is beneficial in adjusting a patient's preoperative psychological state and relieving the mental stress of medical staff during the epidemic.
		2020 Vol. 22 (4): 247-252 [Abstract] ( 319 ) [HTML 1KB] [ PDF 785KB] ( 2656 )

Lecture

	253	Correct Choice of Goggles and Anti-Fog Guidance during the COVID-19 Epidemic Period
		Xiaoqiong Huang1,Jia Qu1,Yanyan Chen1,Peihua Zhang1,Chunmei Zhang2,Xiuyun Zheng3
		During the coronavirus disease 2019 (COVID-19) epidemic period, medical staff do all kinds of effective protection in order to avoid virus infection. Masks, protective clothing and goggles are essential for preventing viral infections. It was found that most goggles worn by the medical staff could fog up quickly. This situation will block vision, increase risks and interfere with clinical work; nurses cannot perform duties effectively, doctors cannot diagnose and treat accurately. Emergency rescue of patients is delayed, and improper protection even leads to cross infection in the hospital. After a literature review and results of clinical practice, combined with professional knowledge, we propose how to choose protective goggles correctly, and seven methods to keep goggles clear of fog. This study is expected to be helpful to medical workers in the prevention and control of COVID-19.
		2020 Vol. 22 (4): 253-255 [Abstract] ( 441 ) [HTML 1KB] [ PDF 701KB] ( 3752 )

Orignal Article

	256	Visual Quality and Reading Performance after Multifocal Intraocular Lens Implantation in Cataract Patients
		Peipei Wei, Wenjuan Wan, Can Li
		Objective: To evaluate the visual quality and reading performance of cataract patients after multifocal intraocular lens implantation. Methods: This was a prospective clinical study. Seventy-five cataract patients (150 eyes) who had cataract surgery in the Department of Ophthalmology, the First Affiliated Hospital of Chongqing Medical University from June 2018 to October 2019 were involved. Twenty-five patients (fifty eyes) were implanted bilaterally with a Tecnis ZCB00 IOL as the monofocal group, 25 patients (fifty eyes) were implanted bilaterally with a Tecnis ZMB00 IOL as the bifocal group and 25 patients (fifty eyes) were implanted bilaterally with an AT Lisa tri.839MP IOL as the trifocal group. The uncorrected distance visual acuity (UDVA), uncorrected intermediate visual acuity (UIVA), uncorrected near visual acuity (UNVA), reading performance at 40 cm and the Chinese NEI-RQL-42 scale were assessed 3 months postoperatively. Data were assessed with a Chi-square test, analysis of variance, Mann-Whitney U test and Kruskal-Wallis test. Results: UDVA showed no significant difference among the three groups (H=3.187, P=0.203); the UIVA of the trifocal group (0.2±0.1) was significantly better than the monofocal group (0.3±0.3)(Z=-2.142, P=0.032) and the bifocal group (0.3±0.2)(Z=-2.041, P=0.041). UNVA was significantly better in the bifocal group (0.3±0.2) and trifocal group (0.3±0.2) than in the monofocal group (0.4±0.2)(t=3.014, P=0.036 and t=2.332, P=0.017, respectively). No statistically significant difference was found in reading acuity at 40 cm (F=0.421, P=0.658), reading speed (F=1.754, P=0.182) or reading mistake rates (H=6.347, P=0.052) among the three groups. Near vision: The bifocal group (100±11) and trifocal group (92±14) were better than the monofocal group (50±42)(U=-3.726, P=0.001 and U=-3.139, P=0.005, respectively); Medium vision: The trifocal group (100±12) was better than the monofocal group (25±75)(U=-3.145, P=0.002); and the trifocal group was better than the bifocal group (75±25)(U=-2.758, P=0.017); Spectacle independence rate: The bifocal group (100±40) and trifocal group (100±25) were better than the monofocal group (30±100)(U=-3.766, P=0.001 and U=-3.004, P=0.008, respectively). Conclusions: Reading performance can evaluate the near visual quality of cataract patients. The AT Lisa tri 839MP IOL can provide distance and near visual acuity and reading performance similar to the Tecnis ZMB00 IOL with better medium visual quality
		2020 Vol. 22 (4): 256-261 [Abstract] ( 394 ) [HTML 1KB] [ PDF 890KB] ( 2952 )

	262	Comparison of Visual Acuity and Visual Quality between Trifocal Intraocular Lens and Bifocal Intraocular Lens Implantation
		Hui Zhong, Hong Qin, Huijuan Wang, Lei Yu, Zhaoyi Wang, Na Li
		Objective: To analyze visual acuity and visual quality after implantation of Zeiss 839 and Zeiss 809. Methods: In this retrospective study, a total of 54 patients (83 eyes) who underwent phacoemulsification were reviewed from August 2017 to January 2019. Twenty-eight patients (42 eyes) were implanted with Zeiss 839 trifocal lenses (839 group), and 26 patients (41 eyes) were implanted with Zeiss 809 bifocal lenses (809 group). Uncorrected visual acuity as well as visual quality were analyzed by the OQAS at 1 week, 1 month and 3 months postoperatively, and the defocus curve and the results of the questionnaire survey at 3 months postoperatively were compared between the two groups. A Paired-sample t test and repeated measures analysis of variance were used to analyze the data. Results: The 839 group had better distance uncorrected visual acuity (UCVA) 1 month and 3 months postoperatively, and better intermediate UIVA at each observation time point than the 809 group (both P<0.05). The OSI, MTF cutoff, SR, PVA100%, PVA20% and PVA9% were not significantly different between the 839 group and 809 group postoperatively. The defocus curve showed that the wave form of the 839 group was more stable and higher in the range of -3 to -1 D. The incidence of glare, light and dark visual adaptation, independent spectacle ratio and satisfaction in the 839 group and 809 group were 2% vs. 7%, 93% vs. 88%, 98% vs. 78%, 90% vs. 85%, respectively. Conclusions: The trifocal IOL and bifocal IOL have excellent uncorrected near and distance vision and superior visual quality. Zeiss 839 has excellent middle distance vision, a higher independent spectacle ratio and better satisfaction.
		2020 Vol. 22 (4): 262-267 [Abstract] ( 401 ) [HTML 1KB] [ PDF 788KB] ( 3108 )

	268	Clinical Effects of Q-Value-Guided Non-Linear Aspherical Monocular LASIK for Myopic and Presbyopic Patients
		Huanjun Su, Ying Lin, Xia Zhang, Muzhi Yuan
		Objective: To evaluate the safety, efficacy and visual outcomes in myopic and presbyopic patients, after Q-value-guided non-linear aspherical monocular LASIK. Methods: A prospective study was performed based on 50 patients (100 eyes) who underwent Q-value-guided non-linear aspherical LASIK. They were recruited in Liuzhou Worker's Hospital from January to December 2018. One week, 1 month, 3 months and 6 months after the operation, the patients' postoperative follow-up included assessments of visual acuity, auto-refraction, Q-value, spherical aberration, contrast sensitivity, corneal topography, stereoacuity and so on. The impact of the operation at 6 months was evaluated with the Chinese quality-of-life impact of refractive correction scale (QIRC). A t-test and repeated measurement analysis of variance were used for data analysis. Results: The demographic information was matched preoperatively between the two eyes. Six months postoperatively, 47 patients achieved an uncorrected distance visual acuity of 0.0 or better in the dominant eye, an uncorrected near visual acuity of 0.3 or better in 48 patients (96%), and 0.33 in the non-dominant eye of all patients. At 6 months, there was a significant difference in Q-value between the dominant eye and the non-dominant eye (t=2.142, P=0.03). Refractive error and Q-value were positively correlated with spherical aberration (r=0.496, P<0.001; r=0.197, P=0.05). At one week postoperatively, all contrast sensitivity spatial frequencies were lower than that before surgery, and gradually reached the preoperative level at 6 months postoperatively (all P<0.05). Most of the patients reported good visual quality, but 2 of them complained of night glare and ghosting. There was no significant difference in stereopsis 6 months postoperatively. Conclusions: Q-value-guided non-linear aspherical monocular LASIK is safe and effective to treat myopia and presbyopia.
		2020 Vol. 22 (4): 268-273 [Abstract] ( 379 ) [HTML 1KB] [ PDF 759KB] ( 2587 )

	274	The Incidence and Characteristics of Suction Loss in Small Incision Lenticule Extraction Procedures
		Wei Chen, Jiabao Chen, Meiping Xu
		Objective: To explore the incidence, characteristics, treatments, and outcomes of suction loss cases during small incision lenticule extraction (SMILE). Methods: In this retrospective non-comparative review of case series studies, 16 eyes of 16 patients occurred suction loss in 3 365 consecutive SMILE procedures between June 2016 and March 2019 in the Shenzhen Hospital of Peking University. They were analyzed for the incidence, the eye, the gender, the locations of suction loss, the instant rotated directions of the eyes during the suction loss, treatments, and prognoses. Data were analyzed using Chi-square test. Results: This study included totally 3 365 eyes (1 692 right eyes, 1 673 left eyes) of 1 698 consecutive patients (517 males, 1 181 females). Suction loss occurred in 16 patients (16 eyes), including 9 right eyes (9/16), 7 left eyes (7/16), from 5 males (5/16) and 11 females (11/16). The incidence of suction loss was 0.46%. There were no significant difference between the loss of aspiration and gender (χ2 =0.005, P=0.944), and eye (χ2 =0.229, P=0.632). Suction loss was located at the laser scanning diameter of 3.62±2.55 mm, mostly within the diameter of 2 mm. The eyes were categorized by suction loss interface and managements: 4 eyes had suction loss in the lenticule interface (1/4), 2 of which were changed to femtosecond laser-assisted laser in-situ keratomileusis (FS-LASIK) immediately, and the other 2 eyes were converted to laser epithelial keratomileusis (LASEK) at once; 1 eye occurred suction loss during lenticule side cut (1/16), and changed to LASEK immediately; 10 eyes had suction loss in the cap interface (5/8), of which 8 eyes promptly redocked and restarted cap and removed the lenticule completely, 1 eye underwent SMILE procedure in 3 weeks later. 1 eye immediately redocked and followed the re-SMILE same cap procedure, in which case, however, another suction loss occurred and the LASEK was performed in 2 weeks later. 1 eye had suction loss in small incision (1/16), and the small incision was cut with a 5 ml injection needle with the lenticule completely removed. The 16 eyes with suction loss were also categorized by the instant rotation directions in suction loss: 9 eyes in upper temporal (9/16), 2 eyes in lower temporal (1/8), 2 eyes in upper nasal (1/8), 2 eyes in lower nasal (1/8) and 1 eye in down (1/16). The uncorrected visual acuity of all the suction loss cases achieved the preoperative best corrected visual acuity 3 months after surgery. Conclusions: Suction loss during SMILE is rare. It mostly occurs in cap interface and near the center of the pupil. Bell's reflexes the main cause of suction loss. Different sites and states of suction loss should have different managements and properstrategies will not affect the long-term postoperative visual acuity.
		2020 Vol. 22 (4): 274-279 [Abstract] ( 346 ) [HTML 1KB] [ PDF 5544KB] ( 2728 )

	280	Comparison of the Effectiveness of Two Visual Training Models for Anisometropic Amblyopia in Patients Aged 9─16
		Yao Zeng1,Quan Lin1, 2,Hang Chu3
		Objective: To compare the effectiveness of visual training based on the binocular push-pull model and traditional combined training for anisometropic amblyopia in patients aged 9 to 16 years. Methods: This was a prospective randomized, controlled study. A total of 64 patients (64 eyes) aged 9 to 16 years who had been diagnosed with anisometropic amblyopia were recruited in Nanning Aier Eye Hospital from June 2017 to March 2018. Patients were randomly divided into a push-pull training group (32 eyes) and a traditional combined training group (32 eyes). The two groups underwent visual training and refractive correction for 12 months. Patients in the push-pull training group were trained with video stimulation by binocular splitting without eye covering, and the training details were designed based on visual acuity (VA), eye position during perception, degree of inhibition, severity of visual impairment and the effect of the most recent training session. Meanwhile, patients in the traditional combined training group underwent fine training and red light flashing at the same time by covering the non-amblyopic eye. The best corrected visual acuity, zero-order stereopsis, first-order stereopsis, second-order stereopsis and refractive error before and after treatment were compared between the two groups. A rank sum test compared the therapeutic effect (grading data), and order of stereopsis between the groups. An independent samples t test compared the change in visual acuity between the groups. Results: After 12 months of treatment, a patient lost in each group. The push-pull training group had basically cured 7 eyes (23%), improved 24 eyes (77%), and failed 0 eyes (0%); the traditional combined training group basically cured 2 eyes (6%), improved 25 eyes (81%), and failed 4 eyes (13%). The differences of the therapeutic effects were statistically significant (Z=-2.490, P=0.013). The logMAR visual acuity total for distance in the push-pull training group improved 146 lines, which was higher than 89 lines for traditional combined training group. Furthermore, compared with the traditional combined training group, zero-order stereopsis, the first-order stereopsis and second-order stereopsis of the push-pull training group improved significantly (Z=-4.861, P<0.001; Z=-3.706, P<0.001; Z=-5.819, P<0.001). However, there was no significant difference in spherical refraction, cylinder refraction, or spherical equivalent between the two groups before and after treatment. Conclusions: Visual training based on the binocular push-pull model is superior to the traditional combined training in improving visual acuity and stereopsis of patients with anisometropic amblyopia aged 9 to 16 years.
		2020 Vol. 22 (4): 280-286 [Abstract] ( 366 ) [HTML 1KB] [ PDF 4561KB] ( 3146 )

	287	Quantitative Analysis of Metamorphopsia in Patients with Idiopathic Macular Holes before and after Surgery
		Qi Chen1,Zaoxia Liu2
		Objective: To observe the type of preoperative metamorphopsia and the degree of improvement in metamorphopsia in patients after vitrectomy for an idiopathic macular hole (IMH), and to analyze the relationship between metamorphopsia and visual acuity after vitrectomy. Methods: This was a prospective, non-randomized controlled study. An analysis was conducted on 29 patients (29 eyes) who were diagnosed with idiopathic macular hole (IMH) in the Second Hospital of Jilin University from December 2017 to September 2018. Patients underwent vitrectomy combined with internal limiting membrane peeling or inversion of the internal limiting membrane flap, and intraocular disinfectant air or silicone oil were used as a filling after treatment. Best corrected visual acuity (BCVA) was assessed before and after surgery. The severity of the metamorphopsia was quantified using M-CHARTS, and vertical metamorphopsia (MV), horizontal metamorphopsia (MH), and mean metamorphopsia scores (M-Score) were measured. Repeated analysis of variance was used to compare the improvement in visual function before and after surgery. An independent samples t test was used to compare the changes in MV and MH at the same stage. Pearson correlation analysis was used to study the correlation between BCVA and metamorphopsia. Results: A total of 22 eyes with a pincushion distortion were measured before surgery, in which 21 eyes had a disease course of less than 6 months. A total of 7 eyes with an unpatterned distortion were measured before surgery. Of these, 2 eyes had a disease duration of less than 6 months. At any stage after surgery, MV was significantly improved compared with preoperative measurements and the difference was statistically significant (F=52.265, P<0.001). There was a significant improvement in MV between 3 months and 6 months postoperatively, and the difference was statistically significant (F=7.879, P=0.009). MH at any stage after surgery was also significantly improved compared with preoperative measurements (F=27.507, P=0.002). There was no significant improvement in MH between 3 months and 6 months postoperatively, and the difference was not statistically significant (F=0.114, P=0.078). There was no significant difference in the preoperative and postoperative stages between MV and MH (P all >0.05). The correlation between M-Score and BCVA (logMAR) did not reach significance 6 months postoperatively (r=0.360, P=0.055). Conclusions: The type of metamorphopsia in IMH patients with less than 6 months' duration is mainly pincushion distortion, and the type of metamorphopsia in IMH patients with more than 6 months' duration is mainly unpatterned distortion. The vitrectomy of IMH significantly reduces metamorphopsia and restores visual function. The improvement in postoperative visual quality in IMH patients cannot be evaluated by BCVA alone. Metamorphopsia can be used as an index to evaluate the outcome of IMH treatment.
		2020 Vol. 22 (4): 287-292 [Abstract] ( 303 ) [HTML 1KB] [ PDF 1097KB] ( 2457 )

	293	Application of Radioactive 125I Seeds Implantation Brachytherapy in the Comprehensive Treatment of Pediatric Orbital Rhabdomyosarcoma
		Xu Pang, Jinpeng Yang, Ke Zhang, Lili Wang, Yu Zhu
		Objective: To observe the effects and complications of radioactive 125I seeds implantation brachytherapy in the comprehensive treatment of pediatric orbital rhabdomyosarcoma. Methods: This was a retrospective case series study. Eight pediatric cases with orbital rhabdomyosarcoma were treated and followed up from January 2013 to January 2017 in the First Affiliated Hospital of Zhengzhou University. All pediatric patients were treated by surgery, and were diagnosed by pathological examination. There were 6 cases with the embryonal type and 2 cases with the acinar type according to the histopathological classification standard developed by WHO. Based on the Intergroup Rhabdomyosarcoma Study Group (IRSG) stages: 1 case was in stage Ⅰ, 3 cases were in stage Ⅱ, and 4 cases were in stage Ⅲ. All children received standard chemotherapy in pediatrics, and radioactive 125I seeds implantation brachytherapy was planned 1 month after resection. The curative effects and radiation complications were observed. Results: The tumors of 4 patients were completely and cleanly removed in the surgeries, and the others were not. Seven cases completed the comprehensive treatments. The 4-year tumor-free survival rate was 100%, except for 1 patient who did not complete the original chemotherapy plan. This patient eventually died due to brain metastases even though the orbital area was controlled. Follow-up was conducted with a median follow-up time of 48 months (37 to 66 months). Puncture complication: There was 1 case with a puncture hemorrhage. The short-term complications of radiotherapy (less than 6 months): There were 5 cases with irritating corneal symptoms and eyelashes and eyebrows missing, and 2 cases with local skin pigmentation. The long-term complications of radiotherapy (after 6 months): There were 5 cases with local skin pigmentation, 5 cases with ocular surface neovascularization and visual loss, 4 cases with subcutaneous atrophy and sclerosis, 3 cases with corneal irritating symptoms, 2 cases with eyelashes and eyebrows missing, and 2 cases with neovascular glaucoma. The eyeball of 1 patient was removed because of corneal ulcer and perforation. All of the pediatric patients had no abnormalities in the orbital or facial bones. The eyeballs remained intact in 6 cases among the 7 surviving children, and visual acuity was not affected in 3 cases. Conclusions: Pediatric orbital rhabdomyosarcoma that is limited can be treated better by surgery, chemotherapy and radioactive 125I seeds implantation brachytherapy, but the unique complications of radioactive 125I seeds need further study.
		2020 Vol. 22 (4): 293-298 [Abstract] ( 334 ) [HTML 1KB] [ PDF 739KB] ( 2682 )

	299	Retinal Ganglion Cell Layer Analysis by Cirrus-HD Optical Coherence Tomography for Ethambutol-Induced Optic Neuropathy
		Wenyan Sheng, Wei Ge, Shuangqing Wu, Lingya Su
		Objective: To investigate the structural injury patterns in retinal ganglion cell injury from ethambutol (EMB) treatment, and the value of Cirrus-HD OCT examination in the early diagnosis of toxic optic neuropathy. Methods: This was a retrospective case-control study. Sixty tuberculosis (TB) patients who took anti-TB drugs containing EMB from January 2018 to March 2019 were recruited. Among them, 6 patients (12 eyes) who were diagnosed with ethambutal-induced optic neuropathy (EON) were included in the EON group, another 43 patients (85 eyes) who had normal visual function after taking the medicine were included in the drug group. Forty-one patients (82 eyes) who matched the age and gender of the drug group were included in control group 1, and 13 patients (26 eyes) who matched the age and gender of the EON group were included in control group 2. The thickness of the retinal nerve fiber layer (RNFL) and the ganglion cell-inner plexiform layer (GCIPL) on Cirrus-HD OCT images were compared with those of healthy controls. Results: No significant changes in RNFL thickness were found in the drug group compared with control group 1. Meanwhile, the ganglion cell layer visibly decreased in the nasal-superior, nasal-inferior, inferior, temporal-superior and the superior sectors compared with control group 1 (t=-3.149, -2.880, -3.816, -3.697, -2.646, -2.231, -2.323, all P<0.05), except for the temporal inferior sector. The p-RNFL thickness of the EON group increased in the nasal and inferior sectors (t=2.452, 2.314, both P<0.05), while the ganglion cell layer thickness decreased in the nasal-superior, nasal-inferior, inferior, temporal-inferior, temporal-superior and the superior sectors compared with the healthy controls in group 2. The differences were significant (t=-2.809, -2.622, -2.806, -2.461, -2.887, -3.478, all P<0.05). Conclusions: CirrusHD OCT can accurately measure the thickness of the GCL layer, and enables more direct observance of damage to retinal ganglion cells. Combined with the measurement of p-RNFL and GCL thickness, it can assist in the early diagnosis of EON.
		2020 Vol. 22 (4): 299-304 [Abstract] ( 395 ) [HTML 1KB] [ PDF 1261KB] ( 2567 )

	305	The Effect of Overexpression of Proteasome PSMB5 on Antioxidative Ability of Human Lens Epithelial Cells
		Tieying Zhang1,Zhaohui Yuan2,Bingsheng Lou2
		Objective: To discuss the antioxidative ability of proteasome subunit β5 (PSMB5) over expression in human lens epithelial cells. Methods: This is an experimental study. Recombinant plasmid pcDNA3.1- PSMB5 was constructed and transfected into a human lens epithelium strain SRA01/04 to form a stable transfection (experimental group), and empty pcDNA3.1 was also transfected into lens epithelial cells at the same time as a control. Two groups of cells were treated with 40 μmol/L H2O2. The nuclear morphology of the two group cells was observed by Hoechst 33342 stain, and cell apoptosis of the two group cells was dectected by flow cytometry. Data was analyzed by a paired t test. Results: Under a 40 μmol/L H2O2 treated environment, Staining by Hoechst 33342 indicated that the nuclei in the control group were significantly shrunken and condensed compared with those of the experimental group. The apoptotic percentages detected by flow cytometry in the experimental group and control group were (9.3±0.9)% and (15.7±1.9)%,and both had increased compared to pre-treatment with H2O2, which were (5.9±0.8)% and (7.1±1.1)%, respectively. The apoptotic percentage in the control group increased to a higher level than in the experimental group (t=3.742, P=0.008). Conclusions: Overexpression of proteasome PSMB5 can effectively improve the antioxidative ability of human lens epithelial cells.
		2020 Vol. 22 (4): 305-309 [Abstract] ( 317 ) [HTML 1KB] [ PDF 955KB] ( 2346 )

Case Report

	310


		2020 Vol. 22 (4): 310-311 [Abstract] ( 330 ) [HTML 1KB] [ PDF 4563KB] ( 2590 )

	312


		2020 Vol. 22 (4): 312-314 [Abstract] ( 294 ) [HTML 1KB] [ PDF 4591KB] ( 2690 )

Review

	315	Research Status in Mechanism of Endotoxin Tolerance in Uveitis
		Yunli Ling1,Hong Lu1, 2
		Endotoxin tolerance, refers to a series of complex and adaptive adjustment reaction produced by the body given small dose of lipopolysaccharide (LPS) in advance. It is a phenomenon of decreased responsiveness of the body or cells to subsequent stimulation. The pretreatment not only can prevent the LPS from causing excessive inflammation in the body, but also can relieve the symptoms of endotoxin-induced uveitis. Therefore, if we can clarify the mechanism and key control factor of endotoxin tolerance in uveitis, we may find new targets for the treatment in uveitis, which is based on endotoxin tolerance.
		2020 Vol. 22 (4): 315-320 [Abstract] ( 307 ) [HTML 1KB] [ PDF 1090KB] ( 2282 )

News

More...

Dwonload

More...

Links

More...

Copyright @ 2013 Chinese Journal of Optometry Ophthalmology and Visual Science
Tel:86-577-86699366 fax: 86-577-86699366 Email:zhysgx@vip.126.com
Supported byBeijing Magtech
Total visits ：